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KMID : 0613820120220060807
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Journal of Life Science
2012 Volume.22 No. 6 p.807 ~ p.814
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Study on the Antioxidative and Physiological Activities of Saururus chinensis Extract
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Kang Chang-Soo
Lee Min-Joo
Park Cheol-Bem
Bang In-Seok
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Abstract
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Saururus chinensis has long been widely used in oriental folk medicines to treat diseases. In the current study, organic solvent fractions obtained from the main methanolic extract of S chinensis were evaluated for their antioxidative and related physiological activities. The antioxidant activity of the fractions was measured using DPPH free radical scavenging activity, increased in a dose-dependent manner, and the ED_50 of the ethyl acetate fractions exhibited a value of 12.84 μg/ml higher than 27.22 μg/ml compared to the BHT. Also, the cell viability of S. chinensis on H₂O₂-induced HDF cell death (IC_50) showed the highest cell viability of 89.39% in 50 μg/ml of ethyl acetate fraction and 67.98% of visible cell survival rate in n-butanolic fraction. Meanwhile, all fractions of the S. chinensis extract led to a slight down regulation of the mRNA expression of fibulin-5, which is related to skin elasticity, and the ethyl acetate fraction having high antioxidant activity showed a markedly inhibitory effect on chick embryonic angiogenesis using the CAM assay. These results suggest that the ethyl acetate fraction of S. chinensis extract could be a good material in therapeutic application for antioxidant and related anti-angiogenesis activities.
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KEYWORD
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Saururus chinensis, antioxidant activity, angiogenesis, CAM assay
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